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  5. Differential Expression Analysis with limma in R

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Exercise

Boxplot of Top2b

Next a sanity check: create a boxplot of the expression level of Top2b to confirm the null mice have lower levels of Top2b expression.

Recall that you made a similar boxplot in Chapter 1 Exercise 4.

Instructions

100 XP

The ExpressionSet object eset with the doxorubicin data has been loaded in your workspace.

  • Find the row that contains the Top2b data by searching the feature data column "symbol" for "Top2b".

  • Create a boxplot using the formula syntax. The expression level should be to the left of the ~, and the genotype variable to the right.

  • Pass the feature data frame row to main to title the plot.