Test for differential expression between 2 groups
Now that you have an ExpressionSet object and a design matrix, you can fit and test your model with limma.
Bu egzersiz
Differential Expression Analysis with limma in R
kursunun bir parçasıdırEgzersiz talimatları
The ExpressionSet object eset with the leukemia data and the design matrix you just created (design) have been loaded in your workspace.
Fit the model coefficients with
lmFit.Calculate the t-statistics with
eBayes.Summarize the results with
decideTests.Since it doesn't make sense to test the intercept term, specify the coefficient
"Diseasestable"when runningdecideTests.
Uygulamalı interaktif egzersiz
Bu örnek kodu tamamlayarak bu egzersizi bitirin.
# Load package
library(limma)
# Fit the model
fit <- ___(eset, ___)
# Calculate the t-statistics
fit <- ___(fit)
# Summarize results
results <- ___(fit[, "___"])
summary(results)