Container di Biostrings

Ora che sai come verificare l'alfabeto di una sequenza, riesci a controllare qual è la classe del container dell'oggetto zikaVirus?

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Introduzione a Bioconductor in R

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Questo esercizio fa parte del corso

Introduzione a Bioconductor in R

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In this chapter, you will get hands-on with Bioconductor. Bioconductor is the specialized repository for bioinformatics software, developed and maintained by the R community. You will learn how to install and use bioconductor packages. You'll be introduced to S4 objects and functions, because most packages within Bioconductor inherit from S4. Additionally, you will use a real genomic dataset of a fungus to explore the BSgenome package.

Exercise 1: Introduction to the Bioconductor Project Exercise 2: Bioconductor version Exercise 3: BiocManager to install packages Exercise 4: The role of S4 in Bioconductor Exercise 5: S4 class definition Exercise 6: Interaction with classes Exercise 7: Introducing biology of genomic datasets Exercise 8: Discovering the yeast genome Exercise 9: Partitioning the yeast genome Exercise 10: Available genomes

Biostrings are memory efficient string containers. Biostring has matching algorithms, and other utilities, for fast manipulation of large biological sequences or sets of sequences. How efficient you can become by using the right containers for your sequences? You will learn about alphabets, and sequence manipulation by using the tiny genome of a virus.

Exercise 1: Introduzione a Biostrings Exercise 2: Esplorare la sequenza del virus Zika Exercise 3: Container di Biostrings

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Exercise 4: Manipolare Biostrings Exercise 5: Gestione delle sequenze Exercise 6: Da un insieme a una singola sequenza Exercise 7: Sottoinsiemi di un insieme Exercise 8: Funzioni comuni per la manipolazione di sequenze Exercise 9: Perché ci interessano i pattern?Exercise 10: Ricerca di un pattern Exercise 11: Trovare i palindromi Exercise 12: Trovare una regione conservata in sei frame Exercise 13: Cercare una corrispondenza

The IRanges and GenomicRanges packages are also containers for storing and manipulating genomic intervals and variables defined along a genome. These packages provide infrastructure and support to many other Bioconductor packages because of their enriching features. You will learn how to use these containers and their associated metadata, for manipulation of your sequences. The dataset you will be looking at is a special gene of interest in the human genome.

Exercise 1: IRanges and Genomic Structures Exercise 2: IRanges Exercise 3: Constructing IRanges Exercise 4: Interacting with IRanges Exercise 5: Gene of interest Exercise 6: From tabular data to Genomic Ranges Exercise 7: GenomicRanges accessors Exercise 8: ABCD1 mutation Exercise 9: Human genome chromosome X Exercise 10: Manipulating collections of GRanges Exercise 11: A sequence window Exercise 12: Is it there?Exercise 13: More about ABCD1 Exercise 14: How many transcripts?Exercise 15: From GRangesList object into a GRanges object

ShortRead is the package for input, manipulation and assessment of fasta and fastq files. You can subset, trim and filter the sequences of interest, and even do a report of quality. An extra bonus towards the last exercises will give you the tools for parallel quality assessment, wink, wink Rqc. Exciting enough, for this you will use plant genome sequences!

Exercise 1: Sequence files Exercise 2: Why fastq?Exercise 3: Reading in files Exercise 4: Exploring a fastq file Exercise 5: Extract a sample from a fastq file Exercise 6: Sequence quality Exercise 7: Exploring sequence quality Exercise 8: Base quality plot Exercise 9: Try your own nucleotide frequency plot Exercise 10: Match and filter Exercise 11: Filtering reads on the go!Exercise 12: Removing duplicates Exercise 13: More filtering!Exercise 14: Multiple assessment Exercise 15: Plotting cycle average quality Exercise 16: Introduction to Bioconductor