Peaks vs background
Now it is time to compare the coverage distribution of peaks, blacklisted regions, and background. In this exercise, you will examine data from chromosome 5.
The binned read counts for peaks, blacklisted regions, and background for this chromosome are available as peak_bins, bl_bins, and
bkg_bins respectively. The score column contains the read counts.
It may take a moment to load all required data and R packages for this exercise. Please be patient.
Bu egzersiz
ChIP-seq with Bioconductor in R
kursunun bir parçasıdırEgzersiz talimatları
- Prepare read counts for plotting by organizing them in data frames.
- Combine the three data frames using
rbind(). - Create a boxplot of the read counts by bin type.
Uygulamalı interaktif egzersiz
Bu örnek kodu tamamlayarak bu egzersizi bitirin.
# Prepare read counts for plotting by organising them in data frames
peak_scores <- data.frame(source="peaks", fragments=___$score)
bl_scores <- data.frame(source="blacklist", fragments=___)
bkg_scores <- data.frame(source="background", fragments=___)
scores <- rbind(___, ___, ___)
# Create a boxplot of the read counts by bin type
ggplot(___, aes(y=fragments, x=source)) + geom_boxplot()