Menjelajahi genom ragi

Anda akan mulai mengeksplorasi genom ragi sendiri menggunakan paket BSgenome.Scerevisiae.UCSC.sacCer3, yang sudah terpasang untuk Anda.

Seperti data lain di R, Anda dapat menggunakan head() dan tail() untuk menelusuri objek yeastGenome. Anda juga dapat membuat subset genom berdasarkan kromosom menggunakan sintaks $ sebagai berikut: object_name$chromosome_name.

Nama-nama kromosom dapat diperoleh menggunakan fungsi names(), dan nchar() dapat digunakan untuk menghitung jumlah karakter dalam suatu sekuens.

Latihan ini adalah bagian dari kursus

Pengantar Bioconductor di R

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Cobalah latihan ini dengan menyelesaikan kode contoh berikut.

# Load the yeast genome
___

# Assign data to the yeastGenome object
yeastGenome <- ___

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Latihan ini adalah bagian dari kursus

Pengantar Bioconductor di R

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In this chapter, you will get hands-on with Bioconductor. Bioconductor is the specialized repository for bioinformatics software, developed and maintained by the R community. You will learn how to install and use bioconductor packages. You'll be introduced to S4 objects and functions, because most packages within Bioconductor inherit from S4. Additionally, you will use a real genomic dataset of a fungus to explore the BSgenome package.

Exercise 1: Pengantar Proyek Bioconductor Exercise 2: Versi Bioconductor Exercise 3: BiocManager untuk memasang paket Exercise 4: Peran S4 dalam Bioconductor Exercise 5: Definisi kelas S4 Exercise 6: Interaksi dengan kelas Exercise 7: Pengenalan biologi dari himpunan data genomik Exercise 8: Menjelajahi genom ragi

Latihan Saat Ini

Exercise 9: Memartisi genom ragi Exercise 10: Genom yang tersedia

Biostrings are memory efficient string containers. Biostring has matching algorithms, and other utilities, for fast manipulation of large biological sequences or sets of sequences. How efficient you can become by using the right containers for your sequences? You will learn about alphabets, and sequence manipulation by using the tiny genome of a virus.

Exercise 1: Introduction to Biostrings Exercise 2: Exploring the Zika virus sequence Exercise 3: Biostrings containers Exercise 4: Manipulating Biostrings Exercise 5: Sequence handling Exercise 6: From a set to a single sequence Exercise 7: Subsetting a set Exercise 8: Common sequence manipulation functions Exercise 9: Why are we interested in patterns?Exercise 10: Searching for a pattern Exercise 11: Finding Palindromes Exercise 12: Finding a conserved region within six frames Exercise 13: Looking for a match

The IRanges and GenomicRanges packages are also containers for storing and manipulating genomic intervals and variables defined along a genome. These packages provide infrastructure and support to many other Bioconductor packages because of their enriching features. You will learn how to use these containers and their associated metadata, for manipulation of your sequences. The dataset you will be looking at is a special gene of interest in the human genome.

Exercise 1: IRanges and Genomic Structures Exercise 2: IRanges Exercise 3: Constructing IRanges Exercise 4: Interacting with IRanges Exercise 5: Gene of interest Exercise 6: From tabular data to Genomic Ranges Exercise 7: GenomicRanges accessors Exercise 8: ABCD1 mutation Exercise 9: Human genome chromosome X Exercise 10: Manipulating collections of GRanges Exercise 11: A sequence window Exercise 12: Is it there?Exercise 13: More about ABCD1 Exercise 14: How many transcripts?Exercise 15: From GRangesList object into a GRanges object

ShortRead is the package for input, manipulation and assessment of fasta and fastq files. You can subset, trim and filter the sequences of interest, and even do a report of quality. An extra bonus towards the last exercises will give you the tools for parallel quality assessment, wink, wink Rqc. Exciting enough, for this you will use plant genome sequences!

Exercise 1: Sequence files Exercise 2: Why fastq?Exercise 3: Reading in files Exercise 4: Exploring a fastq file Exercise 5: Extract a sample from a fastq file Exercise 6: Sequence quality Exercise 7: Exploring sequence quality Exercise 8: Base quality plot Exercise 9: Try your own nucleotide frequency plot Exercise 10: Match and filter Exercise 11: Filtering reads on the go!Exercise 12: Removing duplicates Exercise 13: More filtering!Exercise 14: Multiple assessment Exercise 15: Plotting cycle average quality Exercise 16: Introduction to Bioconductor