Read Alignments
For the RNA-Seq analysis workflow, we obtain the raw FASTQ sequencing files from the sequencing facility. We assess the quality of our sequence reads for each sample, then determine from where on the genome the reads originated by performing alignment. We will use the information about where the reads align to generate the count matrix, which we will be using to start the differential expression analysis. What was quantified to generate the count matrix?
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RNA-Seq with Bioconductor in R
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