Do these samples look the same to you?
Examining PCA plots allows you to get a sense of similarities and differences between samples on a global scale. Using the output generated during sample QC it is straightforward to generate PCA plots of the processed data. Below is a plot for all four samples that passed QC.
Examine the plot and then consider the following statements. Which ones are true?
- The samples cluster by condition.
- A plot like this can be created with the
plotPrincomp()
function. - Using a PCA plot it is easy to see which peaks differ the most between groups.
This exercise is part of the course
ChIP-seq with Bioconductor in R
Hands-on interactive exercise
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