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  3. ChIP-seq with Bioconductor in R

Exercise

Volcano plot

Volcano plots visualize the relationship between the difference between groups (expressed as log fold change) and the p-values of the test comparing the peak intensities. This provides a visual overview of statistically significant results and their effect sizes. DiffBind provides the function dba.plotVolcano() to create these plots.

The ar_diff object contains the results of the differential binding analysis.

Instructions

100 XP
  • Create a volcano plot.
  • Examine the plot. Where are the significant results located?