In the videos, we are exploring gene expression differences between the normal and fibrosis samples of wild-type mice. In these exercises, we will be exploring gene expression between the normal and fibrosis samples from mice over-expressing the smoc2 gene.
When performing differential expression analyses on your own, the DESeq2 vignette will be the first place to search for answers to any questions. In this exercise, explore the DESeq2 vignette and use it to pick the best answer below for the question:
Why do we use unnormalized counts as input for DESeq2?
NOTE: Unnormalized means that the counts were not adjusted for the library size, which is the total number of reads counted per sample.