Do neonicotinoid insecticides have unintended consequences?

As a final exercise in hypothesis testing before we put everything together in our case study in the next chapter, you will investigate the effects of neonicotinoid insecticides on bee reproduction. These insecticides are very widely used in the United States to combat aphids and other pests that damage plants.

In a recent study, Straub, et al. (Proc. Roy. Soc. B, 2016) investigated the effects of neonicotinoids on the sperm of pollinating bees. In this and the next exercise, you will study how the pesticide treatment affected the count of live sperm per half milliliter of semen.

First, we will do EDA, as usual. Plot ECDFs of the alive sperm count for untreated bees (stored in the NumPy array control) and bees treated with pesticide (stored in the NumPy array treated).

This exercise is part of the course

Statistical Thinking in Python (Part 2)

Exercise instructions

Use your ecdf() function to generate x,y values from the control and treated arrays for plotting the ECDFs.
Plot the ECDFs on the same plot.
The margins have been set for you, along with the legend and axis labels. Hit submit to see the result!

Hands-on interactive exercise

Have a go at this exercise by completing this sample code.

# Compute x,y values for ECDFs
x_control, y_control = ____
x_treated, y_treated = ____

# Plot the ECDFs
plt.plot(____, ____, marker='.', linestyle='none')
plt.plot(____, ____, marker='.', linestyle='none')

# Set the margins
plt.margins(0.02)

# Add a legend
plt.legend(('control', 'treated'), loc='lower right')

# Label axes and show plot
plt.xlabel('millions of alive sperm per mL')
plt.ylabel('ECDF')
plt.show()

Edit and Run Code

This exercise is part of the course

Statistical Thinking in Python (Part 2)

IntermediateSkill Level

4.8+

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When doing statistical inference, we speak the language of probability. A probability distribution that describes your data has parameters. So, a major goal of statistical inference is to estimate the values of these parameters, which allows us to concisely and unambiguously describe our data and draw conclusions from it. In this chapter, you will learn how to find the optimal parameters, those that best describe your data.

Exercise 1: Optimal parameters Exercise 2: How often do we get no-hitters?Exercise 3: Do the data follow our story?Exercise 4: How is this parameter optimal?Exercise 5: Linear regression by least squares Exercise 6: EDA of literacy/fertility data Exercise 7: Linear regression Exercise 8: How is it optimal?Exercise 9: The importance of EDA: Anscombe's quartet Exercise 10: The importance of EDA Exercise 11: Linear regression on appropriate Anscombe data Exercise 12: Linear regression on all Anscombe data

To "pull yourself up by your bootstraps" is a classic idiom meaning that you achieve a difficult task by yourself with no help at all. In statistical inference, you want to know what would happen if you could repeat your data acquisition an infinite number of times. This task is impossible, but can we use only the data we actually have to get close to the same result as an infinitude of experiments? The answer is yes! The technique to do it is aptly called bootstrapping. This chapter will introduce you to this extraordinarily powerful tool.

Exercise 1: Generating bootstrap replicates Exercise 2: Getting the terminology down Exercise 3: Bootstrapping by hand Exercise 4: Visualizing bootstrap samples Exercise 5: Bootstrap confidence intervals Exercise 6: Generating many bootstrap replicates Exercise 7: Bootstrap replicates of the mean and the SEM Exercise 8: Confidence intervals of rainfall data Exercise 9: Bootstrap replicates of other statistics Exercise 10: Confidence interval on the rate of no-hitters Exercise 11: Pairs bootstrap Exercise 12: A function to do pairs bootstrap Exercise 13: Pairs bootstrap of literacy/fertility data Exercise 14: Plotting bootstrap regressions

You now know how to define and estimate parameters given a model. But the question remains: how reasonable is it to observe your data if a model is true? This question is addressed by hypothesis tests. They are the icing on the inference cake. After completing this chapter, you will be able to carefully construct and test hypotheses using hacker statistics.

Exercise 1: Formulating and simulating a hypothesis Exercise 2: Generating a permutation sample Exercise 3: Visualizing permutation sampling Exercise 4: Test statistics and p-values Exercise 5: Test statistics Exercise 6: What is a p-value?Exercise 7: Generating permutation replicates Exercise 8: Look before you leap: EDA before hypothesis testing Exercise 9: Permutation test on frog data Exercise 10: Bootstrap hypothesis tests Exercise 11: A one-sample bootstrap hypothesis test Exercise 12: A two-sample bootstrap hypothesis test for difference of means

As you saw from the last chapter, hypothesis testing can be a bit tricky. You need to define the null hypothesis, figure out how to simulate it, and define clearly what it means to be "more extreme" in order to compute the p-value. Like any skill, practice makes perfect, and this chapter gives you some good practice with hypothesis tests.

Exercise 1: A/B testing Exercise 2: The vote for the Civil Rights Act in 1964 Exercise 3: What is equivalent?Exercise 4: A time-on-website analog Exercise 5: What should you have done first?Exercise 6: Test of correlation Exercise 7: Simulating a null hypothesis concerning correlation Exercise 8: Hypothesis test on Pearson correlation Exercise 9: Do neonicotinoid insecticides have unintended consequences?

Current Exercise

Exercise 10: Bootstrap hypothesis test on bee sperm counts

Every year for the past 40-plus years, Peter and Rosemary Grant have gone to the Galápagos island of Daphne Major and collected data on Darwin's finches. Using your skills in statistical inference, you will spend this chapter with their data, and witness first hand, through data, evolution in action. It's an exhilarating way to end the course!

Exercise 1: Finch beaks and the need for statistics Exercise 2: EDA of beak depths of Darwin's finches Exercise 3: ECDFs of beak depths Exercise 4: Parameter estimates of beak depths Exercise 5: Hypothesis test: Are beaks deeper in 2012?Exercise 6: Variation in beak shapes Exercise 7: EDA of beak length and depth Exercise 8: Linear regressions Exercise 9: Displaying the linear regression results Exercise 10: Beak length to depth ratio Exercise 11: How different is the ratio?Exercise 12: Calculation of heritability Exercise 13: EDA of heritability Exercise 14: Correlation of offspring and parental data Exercise 15: Pearson correlation of offspring and parental data Exercise 16: Measuring heritability Exercise 17: Is beak depth heritable at all in G. scandens?Exercise 18: Final thoughts