Exercise

Perform PCA on Seurat object

In scRNA-seq analysis, in order to reduce technical noise and computational time, dimensionality reduction is often used as a preliminary step prior to clustering. Thus, prior to clustering cells of the seuset object, we want to perform PCA to reduce the number of dimensions. To do so, we'll use the Seurat's RunPCA().

seuset is available for you in your workspace.

Instructions 1/2

XP

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Explore the object seuset.
Perform PCA using RunPCA() and assign it back to the seuset object. We want to perform PCA on all the genes of the seuset object, so pc.genes should take the names of all the genes, and do not print the result.

Take Hint (-15 XP)