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Exercise

Create Seurat object

To perform clustering using the package Seurat, we need to create a Seurat object. The goal of this exercise is to create a Seurat object for the mouse epithelium dataset used in the previous chapter using the function CreateSeuratObject().

sce is available in your workspace.

Instructions
100 XP

  • Load Seurat and explore the object sce.

  • Create a Seurat object and assign it to the variable seuset. The argument raw.data takes a SingleCellExperiment object. We log normalize the counts with a fixed scale.factor of 10,000. The meta.data of seuset is the same as the colData of our previous sce object.

  • Scale the counts using ScaleData().

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