Exercise

Create Seurat object

To perform clustering using the package Seurat, we need to create a Seurat object. The goal of this exercise is to create a Seurat object for the mouse epithelium dataset used in the previous chapter using the function CreateSeuratObject().

sce is available in your workspace.

Instructions

100 XP

Load Seurat and explore the object sce.
Create a Seurat object and assign it to the variable seuset. The argument raw.data takes a SingleCellExperiment object. We log normalize the counts with a fixed scale.factor of 10,000. The meta.data of seuset is the same as the colData of our previous sce object.
Scale the counts using ScaleData().

Take Hint (-30 XP)