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Exercise

Normalize

Raw gene expression data is messy, especially since many genes will not be relevant for the system you are studying. After receiving a new data set, the first step is to visualize the data and perform the necessary pre-processing steps.

Instructions
100 XP

The ExpressionSet object eset_raw with the raw Populus data has been loaded in your workspace.

  • Use plotDensities to visualize the distribution of gene expression levels for each sample. Disable the legend.

  • Log transform the measurements and re-visualize.

  • Quantile normalize the measurements with normalizeBetweenArrays and re-visualize.