Exercise

Pre-process features

In the video exercise, you saw that the sample distributions for the doxorubicin study were extremely right-skewed. Thus, the first step you need to take is to pre-process the features: log-transform, normalize, and filter.

Instructions

100 XP

The ExpressionSet object eset_raw with the raw data has been loaded in your workspace. The limma package is loaded.

Log transform the measurements. Use plotDensities to visualize. Label the samples by their genotype.
Quantile normalize the measurements with normalizeBetweenArrays and re-visualize.
Use rowMeans to determine which genes have a mean expression level greater than 0.
Filter the genes (i.e. rows) with the logical vector keep and re-visualize.

Take Hint (-30 XP)