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  3. Differential Expression Analysis with limma in R

Exercise

Pathway enrichment

To better understand the effect of the differentially expressed genes in the doxorubicin study, you will test for enrichment of known biological pathways curated in the KEGG database. Which KEGG pathways are over-represented in the differentially expressed genes for the contrasts "dox_wt" and "interaction"?

Instructions

100 XP

The fitted model object fit2 has been loaded in your workspace. The limma package is already loaded.

  • Extract the entrez Gene IDs from the data frame fit2$genes.

  • Test for enriched KEGG pathways with kegga for the contrast "dox_wt". Set the species to "Mm" for Mus musculus.

  • View the top 5 enriched KEGG pathways with topKEGG.

  • Repeat the pathway enrichment for the contrast "interaction".